Site Directed Mutagenes of COX17 Residues, K46, F50, Y61, and E64
نویسنده
چکیده
This research was performed to determine which amino acid residues were responsible for Cox17 copper binding. Cox17 mutants were transformed and expressed in yeast cells. Site directed mutagens of Cox17 occurred at positions 46 (K), 50 (F), 61(Y), and 64 (E). These mutants were not observed to interfere with copper binding.
منابع مشابه
Yeast cox17 solution structure and Copper(I) binding.
Cox17 is a 69-residue cysteine-rich, copper-binding protein that has been implicated in the delivery of copper to the Cu(A) and Cu(B) centers of cytochrome c oxidase via the copper-binding proteins Sco1 and Cox11, respectively. According to isothermal titration calorimetry experiments, fully reduced Cox17 binds one Cu(I) ion with a K(a) of (6.15 +/- 5.83) x 10(6) M(-1). The solution structures ...
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